The main research area of the Department of Biophysics is analysis and characteristics of the interaction of biologically active compounds with biopolimers (proteins, lipids), cell membranes and whole cells in relation to their potential application in biomedicine.  In our investigations we use a modern biophysical and physicochemical techniques like: fluorescence spectroscopy, electron paramagnetic resonance (EPR), Fourier-transform Infra-Red spectroscopy (FTIR), circular dichroism (CD), zeta potential and calorimetry techniques. Our investigations are aimed to understanding and describing the mechanisms responsible for the interaction of the studied compounds at the cellular and molecular level with an explanation of structural and functional dependencies.

About the department:

Research topics:

Studies of the mechanism of action of new antioxidants isolated from plants growing  in Poland and Central Asia. The specificity of interaction with ROS (reactive oxygen species) and RFA (reactive nitrogen species). Protective action against oxidation of proteins, lipids, GSH, DNA in model and cellular systems. Study of the polyphenols influence on the structure of biological membranes.

Physicochemical characterization of the interaction of selected polyphenols with proteins and lipids. Analysis of protein conformation changes using FTIR spectroscopy, spectrofluorometric assay and circular dichroism. The study , using liposomes, interactions of lipids with ligands by differential scanning calorimetry, Zeta potential measurements,spectrofluorometric probes.

Investigation  of compounds of plant origin as antibacterial agents. The study  of their mechanism of action on bacteria and toxins.

Characterization of polyphenols antitumor activity  at the cellular level (including MTT cytotoxicity assay, mitochondrial potential, level of ROS, concentration of Ca2 + ions,changes in cell membrane fluidity)

Structural - functional analysis of plant sesquiterpenes in the aspect of their ionophore properties. Permeability studies of lipid (BLM) and biological membranes, for ions in combination with the analysis of selectivity.


Chosen projects:

“Evaluation of anti-glycation and antioxidant properties of natural polyphenolic compounds in experimental models of diabetic complications”. Project leader: Prof. dr hab. Maria Zamaraeva (Poland), Dr Ashutosh Kumar (India), Bilateral Indo-Poland Science and Technology cooperation programme2017-2018

 “Estimation of anti-aggregation activity of tannins against alpha-synuclein aggregation“ in the frame of Miniatura-1 project. Project leader: dr Szymon Sękowski, National Science Center, duration: 2017-2018.

“Endemic plant species of Central Asia as a source of biologically active compounds useful in medicine” IncoNet CA Twinning Grants in the frame of HORIZON 2020, Duration: 2015-2016.

 “Physicochemical characterization of biomolecular interactions between hydrolysable 1-O-galloyl-4,6-hexahydroxydiphenoyl-β-D-glucose isolated from Oenothera gigas leafs with liposomes. Project leader: dr Szymon Sękowski, duration: 2012.


Cooperation with international and Polish scientific institutions:

UniversiteLibre de Bruxelles (Brussels , Belgium)

National Institute of Pharmaceutical Education And Research (NIPER) – Hyderabad, (India)

Institute of Bioorganic Chemistry Academy of Sciences of Uzbekistan (Tashkent, Uzbekistan)

Institute of Biophysics and Cell Engineering, Belarusian National Academy of Sciences (Minsk, Belarus)

YankaKupala State University of Grodno, (Grodno, Belarus)

University of Lodz, Department of General Biophysics

Wrocław University of Environmental and Life Sciences, Department of Biophysics


Selected equipment:

Electron Paramagnetic Resonance (EPR), Adani system

Spectrophotometer UV-Vis-NIR, Jasco V-770

Chemiluminometr, LUMIstar Omega, BMG Labtech, Biogenet

Spectrofluorimeter, Perkin-Elmer LS-55B

Laminar Air Flow Chamber



Lectures, laboratories:

Biophysics, Membranology, Molecular Biophysics with nanotechnology, Physicochemical methods in biology, Free Radicals in Biology, Biomedical aspects of the biologically active compounds action.


Selected BSc theses:

Reactive oxygen species and aging

Photodynamic therapy of bacterial infections

Calcium in neurons- from physiology to pathology

Interaction of bacterial toxins with biological membranes


Selected MSc theses:

Spectral analysis of the interaction between the selected tannins and proteins

Biological activity of complexes between gold nanoparticles and tannic acid in relation to cancer cells in vitro

Antioxidant potential of the cranberry extract

Head of the Department
Academic Staff
PhD students

  • A.N. Aralbaeva, A.T. Mamataeva, M.V. Zamaraeva, M.K. Murzakhmetova, Comparative assessment of antioxidant and membrane-protective properties of medicinal plant extracts, International Journal of Biology and Chemistry 2018, 11, 2, 75-82 
  • Sekowski Sz., Terebka M., Veiko A., Lapshina E., Sulkowska U., Zavodnik I.B., Abdulladjanova N., Mavlyanov S., Roszkowska A., Zamaraeva M., Epigallocatechin gallate (EGCG) activity against UV light-induced photo damages in erythrocytes and serum albumin - theoretical and experimental studies, Journal of Photochemistry and Photobiology A: Chemistry 2018, 356, 379-388
  • Ilyich T., Charishnikova O., Sekowski Sz., Zamaraeva M., Cheshchevik V., Dremza I., Cheshchevik N., Kiryukhina L., Lapshina E., Zavodnik I., Ferutinin induces membrane depolarization, permeability transition pore formation, and respiration uncoupling in isolated rat liver mitochondria by stimulation of Ca2+-permeability, The Journal of Membrane Biology 2018, DOI: 10.1007/s00232-018-0032-02.
  • Čaryšnikova O.S., Alimdžanova L.I., Zamaraeva M.W., Biologičeskaâ aktivnost' terpenoidov, vydelennyh iz rastenij roda Ferula, Infekciâ, Immunitet i Farmakologiâ 2017, 3, 248-256
  • Olchowik-Grabarek E., Makarova K., Mavlyanov S., Abdulladjanova N., Zamaraeva M., Comparative analysis of BPA and HQ toxic impacts on human erythrocytes, protective effect mechanism of tannins (Rhus typhina), Environmental Science and Pollution Research 2017, http://doi.org/10.1007/s11356-017-0520-2
  • Sekowski S., Bitiucki M., Ionov M., Zdeb M., Abdulladjanova N., Rakhimov R., Mavlyanov S., Bryszewska M., Zamaraeva M., Influence of valoneoyl groups on the interactions between Euphorbia tannins and human serum albumin, Journal of Luminescence 2018, 194, 170-178, http://dx.doi.org/10.1016/j.jlumin.2017.10.033
  • Sekowski S., Ionov M., Abdulladjanova N., Makhmudov R., Mavlyanov S., Milowska K., Bryszewska M., Zamaraeva M., Interaction of α-synuclein with Rhus typhina tannin - implication for Parkinson's disease, Colloids and Surfaces B: Biointerfaces 2017, 155, 159-165 http://dx.doi.org.10.1016/j.colsurfb.2017.04.007
  • Olchowik-Grabarek E., Mavlyanov S., Abdullajanova N., Gieniusz R., Zamaraeva M., Specificity of hydrolysable tannins from Rhus typhina L. to oxidants in cell and cell-free models, Applied Biochemistry and Biotechnology 2016, DOI: 10.1007/s12010-016-2226-1
  • Bitiucki M., Sękowski Sz. - Nanocząsteczki złota w terapii przeciwnowotworowej, Kosmos 2016, 65, 2(311), 227-234
  • Sekowski S., Tomaszewska E., Soliwoda K., Celichowski G., Grobelny J. - Interactions of hybrid gold-tannic acid nanoparticles with human serum albumin, European Biophysics Journal with Biophysics Letters 2016, DOI: 10.1007/s00249-016-1134-1
  • Sekowski S., Ionov M., Dubis A., Mavlyanov S., Bryszewska M., Zamaraeva M. - Biomolecular interaction of tannin isolated from Oenothera gigas with liposomes, Journal of Membrane Biology 2015, DOI: 10.1007/s00232-015-9858-x,
  • Dubis A., Zamaraeva M.V., Siergiejczyk L., Charishnikova O., Shlyonsky V. - Ferutinin as a Ca2+ complexone: lipid bilayers, conductometry, FT-IR, NMR studies and DFT-B3LYP calculations, Dalton Transactions 2015, 44, 16506-16515, DOI:  10.1039/c4dt03892d
  • Lapshina E.A., Zamaraeva M., Cheshchevik V.T., Olchowik-Grabarek E., Sękowski S., Żukowska I., Golovach N.G., Burd V.N., Zavodnik I.B. 2015. Cranberry flavonoids prevent toxic rat liver mitochondrial damage in vivo and scavenge free radicals in vitro. Cell Biochemistry and Function 2015, DOI: 10.1002/cbf.3104
  • Sękowski S., Ionov M., Kaszuba M., Mavlyanov S., Bryszewska M., Zamaraeva M. 2014. Biophysical studies of interaction between hydrolysable tannins isolated from Oenothera gigas and Geranium sanguineum with human serum albumin. Colloids and Surfaces B: Biointerfaces 123: 623-628.
  • Buczkowski A., Urbaniak P., Belica S., Sękowski Sz., Bryszewska M., Palecz B. 2014. Formation of complexes between PAMAM-NH2 G4 dendrimer and L-?-tryptophan and and L-?-tyrosine in water. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 128: 647-652. 
  • Olchowik-Grabarek E., Święcicka I., Andreeva-Kovaleskaya Z., Solonin A., Bonarska-Kujawa D., Kleszczynska H., Mavlyanov S., Zamaraeva M. 2014. Role of structural changes induced in biological membranes by hydrolysable tannins from sumac leaves (Rhus typhina L.) in their antihemolytic and antibacterial effects. Journal of Membrane Biology 247: 533-540.
  • Hagelgans A., Nacke B., Zamaraeva M., Siegert G., Menschikowski M. 2014. Silibinin down-regulates expression of secreted phospholipase A2 enzymes in cancer cells. Anticancer Research 34: 1723-1730.
  • Zavodnik I.B., Dremza I.K., Cheshchevik V.T., Lapshina E.A., Zamaraewa M. 2013. Oxidative damage of rat liver mitochondria during exposure to t-butyl hydroperoxide. Role of Ca2+ ions in oxidative processes. Life Science 92: 1110-1117.
  • Sękowski Sz., Cheval N., Liszka O., Astachv V., Fowkes C., Fahmi A. 2013. Synthesis and characterisation of hybrid materials based on gold nanoparticles and HBP hyperbranched polyesters: Preparation of "volcano-rings" nanostructures. Colloids and Surfaces A: Physicochem. Eng. Aspects  417: 170-178.
  • Gabrielska J., Sękowski Sz., Żukowska I., Przestalski S., Zamaraeva M. 2013. The modified action of triphenyllead chloride on UVB-induced effects in albumin and lipids. Ecotoxicology and Environmental Safety 89: 36-42. 
  • Olchowik E., Lotkowski K., Mavlyanov S., Abdullajanova N., Jonov M., Bryszewska M., Zamaraeva M. 2012. Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.). Cellular & Molecular Biology Letters 17: 333-348.
  • Ionov M., Gordiyenko N., Zukowska I., Tokhtaeva E., Mareninova O., Baram N., Ziyaev K., Rezhepov K., Zamaraeva M. 2012. Stability and antioxidant activity of gossypol derivative immobilized on N-polyvinylpyrrolidone. International Journal of Biological Macromolecules 51: 908-914.
  • Olchowik E., Sciepuk A., Mavlyanov S., Abdullajanova N., Zamaraeva M. 2011. Antioxidant capacities of polyphenols from Sumac (Rhus typhina L.) leaves in protection of erythrocytes against oxidative damage. Biomedicine & Preventive Nutrition 2: 99-105
  • Ionov M., Klajnert B., Gardikis K., Hatziantoniou S., Palecz B., Salakhutdinov B., Cladera J., Zamaraeva M., Demetzos C., Bryszewska M. 2010. Effectt of amyloid beta peptides Ab1-28 and Ab25-40 on model lipid membranes. J. Therm. Anal. Calorim. 99: 741-747.
  • Żukowska I., Gabrielska J., Przestalski S., Zamaraeva M. 2010. Pro-oxidative effect of organolead induced by UVB radiation. Międzynarodowa Konferencja Naukowa, 23-25 czerwca 2010r., Mińsk, Białoruś: 104-106.
  • Zamaraeva M., Charishnikova O., Saidkhodjaev A., Isidorov V., Granosik M., Różalski M, Watała C. 2010. Calcium mobilization by the plant estrogen ferutinin does not induce blood platelet aggregation. Pharmacol. Rep. 62: 1117-1126.
  • Gabrielska J., Pyrkosz K., Przestalski S., Żukowska I., Zamaraeva M. 2009. Oddziaływanie związków organicznych ołowiu i cyny z albuminą w obecności UVB. Ochrona Środowiska i Zasobów Naturalnych 41: 236-242.
  • Żukowska I., Jaroszuk M., Isidorov V., Osipova S., Zamaraeva M. 2009. Antioxidant and antimicrobial activities of the extract from buds leaves exudates of Populus nigra var. Piramidalis. Acta Biochemica Polonica 55(3): 110.
  • Gabrielska J., Żukowska I., Zamaraeva M., Pyrkosz K., Miśkiewicz J., Przestalski S. 2009. Effect of UVB radiation and organometallic compounds on biomolecules of membranes. 7th EBSA European Biophysics Congress, 11-15.07.2009 Genoa, Italy.
  • Gabrielska J., Żukowska I., Zamaraeva M., Pyrkosz-Biardzka K., Przestalski S. 2009. Wpływ związków metaloorganicznych ołowiu i cyny na albuminę. VIII Międzynarodowa Konferencja Naukowo-Techniczna Warszawa, 24-25.09.2009. Obieg pierwiastków w przyrodzie: Bioakumulacja-Toksyczność-Przeciwdziałanie.
  • Ionov M., Gordiyenko N., Olchowik E., Baram N., Zijaev K., Salakhutdinov B., Bryszewska M., Zmaraeva M. 2009. The immobilization of Gossypol derivative on N-Polyvinylpyrrolidone increases its water solubility and modifies membrane-active properities. J. Med. Chem. 52: 4119-4125.
  • Olchowik E., Mavlanov S., Abdulladjanowa N., Turecka K., Ściepuk A., Zamarajeva M.V. 2008. Antyoksydacyjne właściwości polifenoli z liści sumaka (Rhus typhina). W: Gabrielska J. i Misiak P. (red.) Błony biologiczne: 267-271. Wrocław.
  • Isidorov V., Olchowik E., Vinogorova V. T. 2007. Simplified extraction procedure and gas chromatographic determination of acid compounds in plant material. Chemical Analysis (Warsaw) 52: 423-433.
  • Zamaraeva M. V., Sabirov R. Z., Manabe K., Okada Y. 2007. Ca2+ - dependent glycolysis activation mediates apoptotic ATP elevation in HeLa cells. Biochemical and Biophysical Research Communication 363: 687-693.
  • Zamaraeva M.V., Ionov M. V., Olchowik E., Salakhutdinov B.A., Baram N. I. 2006. Antioxidant activity and membrane effects of water soluble and insoluble derivatives of gossypol. W: Spotkania biogerontologiczne w Nenckim Warszawa, 19 maja 2006, Instytut Biologii Doświadczalnej im. M. Nenckiego PAN, Warszawa.
  • Zamaraeva M.V., Sabirov R.Z., Maeno E., Ando-Akatsuka Y., Bessonova S.V., Okada Y. 2005. Cells Die with increased cytosolic ATP during apoptosis: a bioluminescence study with intracellular luciferase. Cell Death and Differentiation 12: 1390-1397.
  • Beskina O.A., Gabdulkhanova A.G., Miller A.V., Safronova V.G., Dalimov D.N., Zamaraeva M.V. 2003. Lead Diglycyrrhizate and its effect on oxidase activity of neutrophils. Chemistry of Natural Compounds 39, N1: 8-10.
  • Abramov Yu.A., Zamaraeva M.V., Hagelgans A.I., Azimov R.R., Krasilnikov O.V. 2001. The influence of plant terpenoids on permeability of mitochondria and lipid bilayers. Biochim. Biophys. Acta. 1512 (1): 98-118.
  • Przestalski S., Kleszczynska H., Trela Z., Spiak Z., Zamaraeva M.V., Glazyrina N., Gagelgans A. 2000. Direct or inderect influence of triphenyl-lead on activity of Na, K-ATPase. Applied Organometallic Chemistry 14: 432-437.
  • Hordienko N.V., Ionov M.V., Abramov A.Yu., Zamaraeva M.V., Hagelgans A.I., Aripov T.F. 2000. The influence of immunomodulater megosin on thymocytes Ca-homeostas. Journal of Theoretical and Clinical Medicine N1: 38-44.
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