The main research area of the Department of Biophysics is analysis and characteristics of the interaction of biologically active compounds with biopolimers (proteins, lipids), cell membranes and whole cells in relation to their potential application in biomedicine. In our investigations we use a modern biophysical and physicochemical techniques like: fluorescence spectroscopy, electron paramagnetic resonance (EPR), Fourier-transform Infra-Red spectroscopy (FTIR), circular dichroism (CD), zeta potential and calorimetry techniques. Our investigations are aimed to understanding and describing the mechanisms responsible for the interaction of the studied compounds at the cellular and molecular level with an explanation of structural and functional dependencies.
About the department:
Studies of the mechanism of action of new antioxidants isolated from plants growing in Poland and Central Asia. The specificity of interaction with ROS (reactive oxygen species) and RFA (reactive nitrogen species). Protective action against oxidation of proteins, lipids, GSH, DNA in model and cellular systems. Study of the polyphenols influence on the structure of biological membranes.
Physicochemical characterization of the interaction of selected polyphenols with proteins and lipids. Analysis of protein conformation changes using FTIR spectroscopy, spectrofluorometric assay and circular dichroism. The study , using liposomes, interactions of lipids with ligands by differential scanning calorimetry, Zeta potential measurements,spectrofluorometric probes.
Investigation of compounds of plant origin as antibacterial agents. The study of their mechanism of action on bacteria and toxins.
Characterization of polyphenols antitumor activity at the cellular level (including MTT cytotoxicity assay, mitochondrial potential, level of ROS, concentration of Ca2 + ions,changes in cell membrane fluidity)
Structural - functional analysis of plant sesquiterpenes in the aspect of their ionophore properties. Permeability studies of lipid (BLM) and biological membranes, for ions in combination with the analysis of selectivity.
“Evaluation of anti-glycation and antioxidant properties of natural polyphenolic compounds in experimental models of diabetic complications”. Project leader: Prof. dr hab. Maria Zamaraeva (Poland), Dr Ashutosh Kumar (India), Bilateral Indo-Poland Science and Technology cooperation programme2017-2018
“Estimation of anti-aggregation activity of tannins against alpha-synuclein aggregation“ in the frame of Miniatura-1 project. Project leader: dr Szymon Sękowski, National Science Center, duration: 2017-2018.
“Endemic plant species of Central Asia as a source of biologically active compounds useful in medicine” IncoNet CA Twinning Grants in the frame of HORIZON 2020, Duration: 2015-2016.
“Physicochemical characterization of biomolecular interactions between hydrolysable 1-O-galloyl-4,6-hexahydroxydiphenoyl-β-D-glucose isolated from Oenothera gigas leafs with liposomes. Project leader: dr Szymon Sękowski, duration: 2012.
Cooperation with international and Polish scientific institutions:
UniversiteLibre de Bruxelles (Brussels , Belgium)
National Institute of Pharmaceutical Education And Research (NIPER) – Hyderabad, (India)
Institute of Bioorganic Chemistry Academy of Sciences of Uzbekistan (Tashkent, Uzbekistan)
Institute of Biophysics and Cell Engineering, Belarusian National Academy of Sciences (Minsk, Belarus)
YankaKupala State University of Grodno, (Grodno, Belarus)
University of Lodz, Department of General Biophysics
Wrocław University of Environmental and Life Sciences, Department of Biophysics
Electron Paramagnetic Resonance (EPR), Adani system
Spectrophotometer UV-Vis-NIR, Jasco V-770
Chemiluminometr, LUMIstar Omega, BMG Labtech, Biogenet
Spectrofluorimeter, Perkin-Elmer LS-55B
Laminar Air Flow Chamber
Biophysics, Membranology, Molecular Biophysics with nanotechnology, Physicochemical methods in biology, Free Radicals in Biology, Biomedical aspects of the biologically active compounds action.
Selected BSc theses:
Reactive oxygen species and aging
Photodynamic therapy of bacterial infections
Calcium in neurons- from physiology to pathology
Interaction of bacterial toxins with biological membranes
Selected MSc theses:
Spectral analysis of the interaction between the selected tannins and proteins
Biological activity of complexes between gold nanoparticles and tannic acid in relation to cancer cells in vitro
Antioxidant potential of the cranberry extract
Wyślij je na stronę internetową Wydziału Biologiczno-Chemicznego.